Highlights
- Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
- DNA-Free: Genomic DNA removal column and DNase I included.
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Description
| Equipment | Microcentrifuge, vortex |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™. |
| Size Range | Total RNA ≥ 17 nt |
| Supplemental Info | |
| Yield | 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q2: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q3: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q4: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q5: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q6: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.
Q7: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q8: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q9: What is the average RNA yield? (chart)
| Input | Average RNA Yield |
|---|---|
| Cells | 10 µg (per 106 cells) |
| HeLa | 15 µg |
| High Yield Tissue (mouse) | ≥ 30 µg (per 10 mg) |
| Spleen | 30-50 µg |
| Liver | 40-60 µg |
| Low Yield Tissue (mouse) | ≤ 30 µg (per 10 mg) |
| Brain, Heart | 5-15 µg |
| Muscle | 5-20 µg |
| Lung | 10-20 µg |
| Intestine | 10-30 µg |
| Kidney | 20-30 µg |
| Whole Blood | (per 1 ml) |
| Porcine | 10-20 µg |
| Human | 2-10 µg |
Q10: How to improve purity, RIN scores and eliminate contamination (i.e., A260/230, A260/280 ratios, DNA, phenol, protein, salt, etc.)?
Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).
Q11: How to improve RNA yield?
- To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
- Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).
"RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."
- Lisa G. (University of Virginia)
“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”
- Erin B. (Occidental College)
"Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."
- Xiaolu J. (Florida Atlantic University)
Read More
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1010-1-16 | DNA Digestion Buffer | 16 mL | $31.90 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $16.50 | |
| R1060-1-100 | RNA Lysis Buffer | 100 ml | $166.10 | |
| R1060-1-50 | RNA Lysis Buffer | 50 ml | $86.90 | |
| R1003-3-48 | RNA Wash Buffer | 48 ml | $115.50 | |
| R1003-3-24 | RNA Wash Buffer | 24 ml | $64.90 | |
| R1060-2-100 | RNA Prep Buffer | 100 ml | $134.20 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | $44.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $16.50 | |
| C1006-50-F | Spin-Away Filters | 50 Pack | $60.50 | |
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | $60.50 | |
| W1001-6 | DNase/RNase-Free Water | 6 ml | $16.50 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $24.20 | |