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    Quick-RNA Miniprep Kit

    R1054 / R1055

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    Quick-RNA Miniprep Kit

    Get Free Sample
    Cat # Name Size Price Quantity
    R1054 Quick-RNA Miniprep Kit 50 preps $229.90
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    R1055 Quick-RNA Miniprep Kit 200 preps $742.50
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    Highlights
    • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
    • DNA-Free: Genomic DNA removal column and DNase I included.
    • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
    Description

    The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.

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    Equipment Microcentrifuge, vortex
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.
    Size Range Total RNA ≥ 17 nt
    Supplemental Info
    Yield 100 µg RNA (binding capacity), ≥50 µl (elution volume)

    Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

    Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

    Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    Input Average RNA Yield
    Cells 10 µg (per 106 cells)
    HeLa 15 µg
    High Yield Tissue (mouse) ≥ 30 µg (per 10 mg)
    Spleen 30-50 µg
    Liver 40-60 µg
    Low Yield Tissue (mouse) ≤ 30 µg (per 10 mg)
    Brain, Heart 5-15 µg
    Muscle 5-20 µg
    Lung 10-20 µg
    Intestine 10-30 µg
    Kidney 20-30 µg
    Whole Blood (per 1 ml)
    Porcine 10-20 µg
    Human 2-10 µg

    Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

    • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
    • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).

    Cells: Pellet by centrifugation (500 x g - 5,000 x g) and remove RNAlater™ (supernatant). Proceed to Sample Preparation, see protocol.

    Tissue: Transfer into a new tube with forceps and remove any excess RNAlater™. Proceed to Sample Preparation, see protocol.

    Alternatively, for liquid samples from which RNAlater™ cannot be removed, add 1 volume of nuclease-free water (or PBS) to 1 volume liquid sample (1:1) and mix. Then add 4 volumes RNA Lysis Buffer to 1 volume sample/water (or PBS) mixture (4:1). Mix again and proceed to Total RNA Purification, see protocol.

    "RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

    - Lisa G. (University of Virginia)

    “Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

    - Erin B. (Occidental College)

    "Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

    - Xiaolu J. (Florida Atlantic University)

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    Cat # Name Size Price
    E1010-1-16 DNA Digestion Buffer 16 mL $31.90
    E1010-1-4 DNA Digestion Buffer 4 mL $16.50
    R1060-1-100 RNA Lysis Buffer 100 ml $166.10
    R1060-1-50 RNA Lysis Buffer 50 ml $86.90
    R1003-3-48 RNA Wash Buffer 48 ml $115.50
    R1003-3-24 RNA Wash Buffer 24 ml $64.90
    R1060-2-100 RNA Prep Buffer 100 ml $134.20
    R1060-2-25 RNA Prep Buffer 25 ml $44.00
    C1001-50 Collection Tubes 50 Pack $16.50
    C1006-50-F Spin-Away Filters 50 Pack $60.50
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack $60.50
    W1001-6 DNase/RNase-Free Water 6 ml $16.50
    W1001-30 DNase/RNase-Free Water 30 ml $24.20