Quick-RNA Microprep Kit

R1050 / R1051


Quick-RNA Microprep Kit

R1050 / R1051

Cat # Name Size Price Quantity
R1050 Quick-RNA Microprep Kit 50 preps $209.00
+ -
R1051 Quick-RNA Microprep Kit 200 preps $675.00
+ -

Documents


Data Sheet: PDF   |   Protocol: PDF   |   SDS (MSDS) - R1050: PDF   |   SDS (MSDS) - R1051: PDF   |  
Isolate total RNA and small/miRNA from cells and soft tissue

Highlights


  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • DNA-Free: Genomic DNA removal column and DNase I included.
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.

Description


The Quick-RNA Microprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™ Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.

Technical Specifications


Equipment Microcentrifuge, vortex
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.
Size Range Total RNA ≥ 17 nt
Yield Microprep: 10 µg RNA (binding capacity), ≥6 µl (elution volume)

Product FAQ


Q1: Is DNase I available for individual purchase?

Q2: Is the DNase-I treatment necessary?

Q3: Is it possible to extract proteins with the Quick-RNA kit?

Q4: How to store DNase-I following resuspension?

Q5: Will the kit isolate small RNAs?

Q6: Can samples be stored in RNA Lysis Buffer prior to processing?

Q7: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q8: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?

Q9: I ran out of RNA Wash Buffer. Can I use something else?

Citations


In a study that compared the ability of six commercially available RNA purification kits to extract miRNA from <em>Gyrodactylus salaris</em>, a parasite of freshwater fish such as the Atlantic salmon, the Quick-RNA MicroPrep kit from Zymo Research was shown to result in both the highest yield of miRNA and the best miRNA/total RNA ratio <em>(note: the Quick-RNA MicroPrep kit is an updated version of the ZR RNA MicroPrep kit, which was used in this study)</em>.  The kit from Zymo Research outperformed the mirVana Kit (Ambion) and the Trizol Plus Kit (Invitrogen). 

Fromm B, Harris PD, and Bachmann L. (2011) MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits. BMC Res Notes. 4:217.

Reviews


"RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

- Lisa G. (University of Virginia)

“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

- Erin B. (Occidental College)

"Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

- Xiaolu J. (Florida Atlantic University)

Read More

Kit Components