Quick-RNA Miniprep Kit

R1054 / R1055


Quick-RNA Miniprep Kit

R1054 / R1055

Cat # Name Size Price Quantity
R1054 Quick-RNA Miniprep Kit 50 preps $209.00
+ -
R1055 Quick-RNA Miniprep Kit 200 preps $675.00
+ -

Documents


Isolate total RNA and small/miRNA from cells and soft tissue

Highlights


  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • DNA-Free: Genomic DNA removal column and DNase I included.
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.

Description


The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.

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Technical Specifications


Equipment Microcentrifuge, vortex
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.
Size Range Total RNA ≥ 17 nt
Yield 100 µg RNA (binding capacity), ≥50 µl (elution volume)

Product FAQ


Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?

Q2: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q3: Can samples be stored in RNA Lysis Buffer prior to processing?

Q4: Will the kit isolate small RNAs?

Q5: I ran out of RNA Wash Buffer. Can I use something else?

Q6: Is it possible to extract proteins with the Quick-RNA kit?

Q7: Is the DNase-I treatment necessary?

Q8: How to store DNase-I following resuspension?

Q9: Is DNase I available for individual purchase?

Citations


Reviews


"RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

- Lisa G. (University of Virginia)

“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

- Erin B. (Occidental College)

"Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

- Xiaolu J. (Florida Atlantic University)

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Kit Components