Plasmid Purification Kits

Plasmid purification is a cornerstone of many molecular biology applications including cloning, recombinant protein expression, transfection, DNA sequencing, gene editing, and in vitro transcription. These applications require high yields of ultra-pure plasmid DNA that can ideally be recovered in a fast and simple protocol. Zymo Research provides unrivaled plasmid purification kits for the rapid recovery of ultra-pure plasmid DNA. Several formats of plasmid purification kits are available, including spin-column, 96-well, and magnetic bead-based kits for isolating bacterial plasmids, yeast plasmids, or BAC, YAC, & PAC DNA. Eluted plasmid DNA is ultra-pure and ready for all sensitive downstream applications. Need help finding the right plasmid purification kit? Check out our Product Comparison Table.

Plasmid from E. coli

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Plasmid from Yeast

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BAC/PAC/YAC DNA

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Plasmid Purification Kit Comparison Tables

Zymo Research offers a variety of plasmid purification kits in different scales and formats for E. coli that are highly efficient and simple to use. All our plasmid purification kits recover transfection-grade plasmid DNA that is suitable for the most sensitive molecular biology techniques, such as restriction digestion, PCR, sequencing and cloning. Use the tables below to find the right plasmid purification kit for you. If you are unsure about your plasmid DNA needs, then the ZymoPURE Miniprep Kit is a good starting point because of its versatility.

Miniprep Plasmid Purification Kits

Kit	Direct Lysis^†	Desired Processing Volume	Desired Plasmid Yield	Desired Plasmid Concentration	Desired Endotoxin Levels (EU/µg plasmid)	Plasmid Size	Processing Time
ZymoPURE Miniprep	No	1 — 5 ml	1 — 100 µg	500 — 1,000 ng/µl	≤ 1 EU/µg	≤ 200 kb	15 min
ZymoPURE 96 Plasmid Miniprep Kit	No	1 — 5 ml	1 — 100 µg	100 — 1,000 ng/µl	≤ 1 EU/µg	≤ 200 kb	60 min
ZR Plasmid Miniprep - Classic	No	1 — 5 ml	< 20 µg	100 — 500 ng/µl	≤ 50 EU/µg	≤ 25 kb	15 min
Zyppy Plasmid Miniprep Kit	Yes	< 1 ml	1 — 3 µg	≤ 100 ng/µl	≤ 50 EU/µg	≤ 25 kb	8 min
Zyppy-96 Plasmid Kit	Yes	< 1 ml	1 — 3 µg	≤ 100 ng/µl	≤ 50 EU/µg	≤ 25 kb	45 min
Zyppy-96 Plasmid MagBead Miniprep	Yes	< 1 ml	1 — 3 µg	≤ 100 ng/µl	≤ 50 EU/µg	≤ 25 kb	45 min
ZR BAC DNA Miniprep	No	1 — 5 ml	1 µg	≤ 50 ng/µl	≤ 50 EU/µg	≤ 200 kb	15 min
_{^†Direct lysis allows you to omit traditional cell pelleting and resuspension steps by lysing the cells directly in the overnight culture media.}

Midi, Maxi & Giga Prep Plasmid Purification Kits (Large Scale)

Kit	Direct Lysis^†	Desired Processing Volume	Desired Plasmid Yield	Desired Plasmid Concentration	Desired Endotoxin Levels (EU/µg plasmid)	Plasmid Size	Processing Time
ZymoPURE Express Midiprep	Yes	25 ml	100 — 200 µg	0.5 — 1 µg/µl	≤ 1 EU/µg	≤ 200 kb	15 min
ZymoPURE II Plasmid Midiprep	No	≤ 50 ml	200 — 400 µg	≥ 1 µg/µl	≤ 0.025 EU/µg	≤ 200 kb	18 min
ZymoPURE II Plasmid Maxiprep	No	≤ 150 ml	600 — 1,200 µg	≥ 1 µg/µl	≤ 0.025 EU/µg	≤ 200 kb	< 18 min
ZymoPURE II Plasmid Gigaprep	No	1 — 2.5 L	3,000 — 20,000 µg	≥ 1 µg/µl	≤ 0.025 EU/µg	≤ 200 kb	< 45 min
_{^†Direct lysis allows you to omit traditional cell pelleting and resuspension steps by lysing the cells directly in the overnight culture media.}

Need help finding the right plasmid purification kit? Click here to compare kits.