Description
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
Technical Specifications
| Applicable For |
For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
| Elution Volume |
25 µl |
| Equipment |
Microcentrifuge, vortex, cell disrupter/pulverizer |
| Processing Time |
≤ 10 minutes |
| Processing Volume |
50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells |
| Purity |
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8. |
| Sample Source |
Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range |
Total RNA ≥ 17 nt |
| Type |
Total RNA |
| Yield |
Miniprep
50 µg RNA (binding capacity)
≥ 25 µl (elution volume) |
Kit Components
| Cat # |
Name |
Size |
Price |
|
|
W1001-1
|
DNase/RNase-Free Water
|
1 ml
|
$10.00 |
|
|
R1003-3-24
|
RNA Wash Buffer
|
24 ml
|
$59.00 |
|
|
R1060-1-50
|
RNA Lysis Buffer
|
50 ml
|
$79.00 |
|
|
R1060-2-25
|
RNA Prep Buffer
|
25 ml
|
$40.00 |
|
|
C1001-50
|
Collection Tubes
|
50 Pack
|
$15.00 |
|
|
C1006-50-G
|
Zymo-Spin IIICG Columns
|
50 Pack
|
$55.00 |
|
|
C1011-50
|
Zymo-Spin IIC Columns
|
50 Pack
|
$55.00 |
|
|
S6012-50
|
ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)
|
50 Tubes
|
$101.00 |
|
