Quick-RNA Fungal/Bacterial Miniprep Kit

R2014


Quick-RNA Fungal/Bacterial Miniprep Kit

R2014

Cat # Name Size Price Quantity
R2014 Quick-RNA Fungal/Bacterial Miniprep Kit 50 preps $255.00
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Documents


Protocol: PDF   |   SDS (MSDS): PDF   |  
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Highlights


  • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
  • Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
  • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.

Description


The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.

Technical Specifications


Applicable For For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Elution Volume 25 µl
Equipment Microcentrifuge, vortex, cell disrupter/pulverizer
Processing Time ≤ 10 minutes
Processing Volume 50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8.
Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
Size Range Total RNA ≥ 17 nt
Type Total RNA
Yield Miniprep
50 µg RNA (binding capacity)
≥ 25 µl (elution volume)

Kit Components