Quick-RNA Fungal/Bacterial Miniprep Kit
R2014
Highlights
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
Description
| Applicable For | For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
|---|---|
| Elution Volume | 25 µl |
| Equipment | Microcentrifuge, vortex, cell disrupter/pulverizer |
| Processing Time | ≤ 10 minutes |
| Processing Volume | 50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells |
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range | Total RNA ≥ 17 nt |
| Type | Total RNA |
| Yield | Miniprep 50 µg RNA (binding capacity) ≥ 25 µl (elution volume) |
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| W1001-1 | DNase/RNase-Free Water | 1 ml | $11.00 | |
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | $60.50 | |
| C1001-50 | Collection Tubes | 50 Pack | $16.50 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | $44.00 | |
| R1003-3-24 | RNA Wash Buffer | 24 ml | $64.90 | |
| R1060-1-50 | RNA Lysis Buffer | 50 ml | $86.90 | |
| R1060-1-100 | RNA Lysis Buffer | 100 ml | $166.10 | |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | $111.10 | |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $60.50 | |