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    ZymoBIOMICS DNA Miniprep Kit Sample


    Cat # Name Size Price Quantity
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    Highlights

    • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
    • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
    • Certified Low Bioburden: Boost your detection limit for low abundance microbes.
    Description & Documents Specifications Components

    Documents

    Product Description

    The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

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    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 50 µl DNA Elution Buffer or DNase free water
    Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
    Processing Volume Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells)
    Purity High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
    Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
    Sample Storage Eluted DNA should be stored at ≤ -20°C.
    Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
    Supplemental Info
    Type Total DNA
    Yield Up to 25 µg total DNA can be eluted into ≥50 µl
    Supplemental Info

    Resources

    Cat # Name Size Price
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack $122.40
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes $114.40
    C1078-50 Zymo-Spin IICR Columns 50 Pack $62.30
    D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml $45.30
    D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml $156.40
    D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml $52.10
    D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml $111.00
    D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml $36.30
    D4302-5-1 ZymoBIOMICS DNase/RNase Free Water 1 ml $11.30
    D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml $31.70
    C1057-50 Zymo-Spin III-F Filters 50 Pack $66.80

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