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Highlights
- Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
Original Manufacturer
100% satisfaction guaranteed, read Our Promise
Innovated in California, Made in the USA
Highlights
- Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
Original Manufacturer
100% satisfaction guaranteed, read Our Promise
Innovated in California, Made in the USA
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $64.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $18.00 | |
| D5002-4 | M-Wash Buffer | 24 ml | $33.00 | |
| D5001-4 | M-Wash Buffer | 6 ml | $13.00 | |
| D5005-3 | M-Binding Buffer | 30 ml | $26.00 | |
| D5002-6 | M-Elution Buffer | 4 ml | $13.00 | |
| D5001-6 | M-Elution Buffer | 1 ml | $13.00 | |
| D5006-3 | M-Binding Buffer | 125 ml | $76.00 | |
| D5030-5 | L-Desulphonation Buffer | 10 ml | $20.00 | |
| D5031-5 | L-Desulphonation Buffer | 40 ml | $55.00 | |
| D5030-1 | Lightning Conversion Reagent | 1.5 ml | $30.00 | |
Description
Performance
Technical Specifications
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. |
|---|---|
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 µl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100 pg - 2 µg of DNA. |
| Processing Time | 1.5 hours |
| Recovery | > 80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
Resources
Documents
FAQ
ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).
Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and subsequently result in lower yields.
Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.
> 50 bp.
Following bisulfite treatment of genomic DNA, nonmethylated cytosine residues are converted into uracil. The recovered DNA is typically A, U, and T-rich. The original base-pairing no longer exists. Instead, it is single-stranded with limited non-specific base-pairing at room temperature. The absorption coefficient at 260 nm resembles that of RNA. Use a value of 40 μg/ml for Ab260 = 1.0 when determining the concentration of the recovered bisulfite-treated DNA. To visualize, run converted DNA on agarose gel then chill the gel on ice for 30 minutes. The expected smear will be between 100-1500bp.
Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.
Converted DNA should be eluted in M-Elution Buffer to keep the converted DNA stable for long term storage. If stored properly for long term (<-20C), the samples should last longer than a month. Minimize freeze/thawing to keep the bisulfite converted DNA stable.
Product Video
Reviews
"Results were as good as with the formerly used EZ DNA Methylation Gold Kit but significantly reduced total time for analyses. More flexibility due to the possibility of up to 20h storage of the samples after bisulfite conversion before the cleaning procedure especially for part-time employees."
Sabrina S.
UK-Aachen
"The conversion reagent no longer has to be made up from individual components and dissolved - it is supplied ready-made. The conversion reagent can be stored at room temperature - there is no need to use up all 10 uses in one month. This makes it more flexible, and avoids wastage. Shorter protocol saves time."
Jeremy B.
AgResearch
Citations
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