Epigenetics

The Zymo-Seq Trio WGBS Library Kit offers an optimized and reliable workflow for whole genome bisulfite sequencing (WGBS) library preparation from cell-free DNA (cfDNA), FFPE DNA, and gDNA.

Zymo-Seq WGBS Library Kit is the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube.

The EZ DNA Methylation-Lightning Automation Kit is a high-throughput DNA bisulfite conversion kit designed for streamlining automation workflows.

The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up...

The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite...

The EZ DNA Methylation-Lightning magbead based Kits are the fastest magnetic bead-based DNA bisulfite conversion kit for high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent - simply...

The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite...

The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of...

The EZ-96 DNA Methylation-Direct MagPrep is a magnetic bead-based bisulfite conversion kit that allows simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity...

The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. This kit is the original bisulfite conversion kit from Zymo Research. The EZ DNA Methylation Kit is based on the three-step reaction that takes place...

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during ...

The EZ-96 DNA Methylation MagPrep is a bisulfite conversion kit that features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine...

The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into...

The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to...

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is ...

The Pico Methyl-Seq Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The...

Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation...

The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a...

Enzymatic Real-Time PCR for the detection of DNA methylation.

The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation...

The mouse Anti-5-Methylcytosine Monoclonal Antibody specifically recognizes methylated cytosines. The antibody binds to 5-mC in single-stranded DNA, with no detectable cross-reactivity to non-methylated or hydoxymethylated cytosines. This product is ...

The Universal Methylated DNA Standards are designed to be used as positive controls to assess the efficiency of DNA bisulfite conversion in human and mouse models. The control DNA can be run in parallel with samples to monitor the bisulfite conversion...

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ ...

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation...

The Bisulfite-Converted Universal Methylated Human DNA Standard is designed to be used as a control for DNA bisulfite conversion and downstream analyses including PCR, MSP, and other amplification-based assays. This DNA is identical to our Universal ...

E. coli non-methylated genomic DNA is from a Dam- and Dcm- strain (ER2925) of E. coli. It works perfectly as a negative control for DNA methylation analyses requiring DNA with absolutely no methylation.

The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a “spike-in” control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce...

The Human Matched DNA Set is a set of organ specific human genomic DNAs originating from a single individual. This set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified...

The Mouse 5-hmC & 5-mC DNA Set is a set of organ specific mouse genomic DNAs isolated from a pool of 8-10 week old Swiss Webster mice. This genomic DNA set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine...

The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three standards that are linear dsDNA at a length of 897 bp. All DNA standards have the same sequence (see protocol for sequence), the only difference being that each standard...

Zymo-Seq Methyl Spike-in Control is comprised of six unique double-stranded synthetic amplicons (180-200 bp) with distinct sequences derived from the E. coli K12 genome, and each amplicon represents a different CpG methylation level ranging from 0% to...

The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to ...

ZymoTaq PreMix is supplied as a 2X concentrated “master mix” containing all the reagents needed to perform “hot start” PCR. The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that ...

ZymoTaq DNA Polymerase contains all the reagents needed to perform “hot-start” PCR. The inclusion of a heat-activated, thermostable DNA polymerase reduces primer dimer and nonspecific product formation that can occur during PCR. This unique product is...

ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays. This premix features a hot-start...

The Quest 5-hmC DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as...

The rabbit Anti-5-hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross-reactivity. The antibody has been validated in ELISA and immunoprecipitation-based enrichment assays,...

The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI...

DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation...

The Zymo-Spin ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been “fixed” in their natural state and can be used to effectively identify binding sites for transcription factors, ...

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse...

The ZR-96 Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid, high-throughput purification, and concentration of high-quality DNA from any step in a standard ChIP protocol. This product is well suited...

The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification....

Zymo-Seq ATAC Library Kit provides a simple and streamlined workflow for Assay for Transposase Accessible Chromatin with high throughput Sequencing (ATAC-Seq) library preparation from mammalian cell and tissue input. This all-inclusive kit features an...

DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.)....

Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.

Cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3'-phosphates.

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired...

CpG Methylase (M. SssI) ensures complete, in vitro methylation of DNA for methylation analysis.

5-hmC detection; 5-hmC enrichment

Modified deoxynucleotide for use with PCR, mass spec, etc.

dNTP mixture containing the modified deoxynucleotide 5-mC for use with PCR, mass spec, etc.

dATP solution for use in nucleotide mixes, PCR, RT-PCR, library preps, etc.

Ultra pure dCTP can be used in nucleotide mixtures to generate PCR amplicons using ZymoTaq, QuestTaq or other DNA polymerases.

dATP solution for use in nucleotide mixes, PCR, RT-PCR, library preps, etc.

dTTP solution for use in nucleotide mixes, PCR, RT-PCR, library preps, etc.

dGTP solution for use in nucleotide mixes, PCR, RT-PCR, library preps, etc.